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The 2nd response cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to produce ferric enzyme, NO, citrulline, and water as products (Fig. 9) - UV/Vis. As attracted Fig - https://pxhere.com/en/photographer/4182440. 9, the reaction might also include electron transfer from H4B to the Fe, IIO2 intermediate
l9 As shown in Fig. 9, this could be because the H4B extreme acts as an electron sink in a later point in the response, so as to permit NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.
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